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Putrescine (3TMS)

 

Replica Mass Spectra of Putrescine (3TMS)

replicalib entry datedetectionmethodspecies
201 November 2001 MS-QuadM[2]Reference Substance
515 March 2005 MS-TOFM[6]Reference Substance
601 May 2001 MS-QuadM[2]Standard
701 May 2001 MS-TOFM[EIGTMS]Standard Addition
1105 June 2013  MRI_2013 
801 January 2003 MS-QuadM[2]Standard
6 spectrum(a)
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Spectrum Details

analytePutrescine (3TMS)
analyte InChIInChI=1S/C13H36N2Si3/c1-16(2,3)14-12-10-11-13-15(17(4,5)6)18(7,8)9/h14H,10-13H2,1-9H3
analyte mass304.7
chromatogram6018if_3
citationNA
authorsErban A, Strehmel N, Kopka J, Max Planck Institute of Molecular Plant Physiology, Department of Molecular Plant Physiology (Prof. Willmitzer L), Am Muehlenberg 1, D-14476 Golm, Germany
lib entry date14 April 2010
metabolite role 
retention time (sec)821.49
retention index (VAR5 method, n-alkanes C10–C36)1.506,15 (observed)
base peak (m/z)73
maximal intensity102.934
mass-intensity-peaks cardinality140 intensities
minimal m/z45
maximal m/z907
download JCAMP DXSpectrumJcampDx.ashx?id=209eed23-4c65-40a9-be9c-2c3e9391a601
download MSPSpectrumMsp.ashx?id=209eed23-4c65-40a9-be9c-2c3e9391a601

GC-Method

nameVAR5
citationKopka J, Max Planck Institute of Molecular Plant Physiology, Department of Molecular Plant Physiology (Prof. Willmitzer L), Am Muehlenberg 1, D-14476 Golm, Germany
attributetextdetails
deconvolutionChromaTOFOFFSET: "just above noise"; SMOOTHING: 20; PEAK WIDTH: 6; S/N: 2
derivatizationMEOX; TMSMETHOXYAMINATION: 90min at 30°C; TRIMETHYLSILYLATION: 30min at 37°C
detectorMS-TOFm/z = 70-600; scans:20/s; Pegasus III TOF mass spectrometer
extractionwater:chloroform (2:1; v/v) 15min at 70°C
gas chromatographAgilent GC 6890 
ion sourceEI70eV
mass spectrometry instrumentPegasus III TOF mass spectrometer 
metabolic inactivationshock-frozen in liquid nitrogen 
RIALKANEC10, C12, C15, C18, C19, C22, C28, C32, C36
separationGCCOLUMN:5%phenyl-95%dimethylpolysiloxane, 30+10m, ID:0.25mm, DF:0.25µm, 5PDM VF-5ms (Varian, Darmstadt, Germany); PROGRAM:iso1min 70°C, ramp 9°C/min, iso 5min 350°C; FLOW:Helium, 0.6mL/min; INJECTION:1µL, splitless, 230°C; TRANSFER:250°C; IONSOURCE:250°C
protocolpropertyvalue
extractionwater chloroform(2:1; v/v) 15min at 70°C
derivatizationderivatisationMETHOXYAMINATION, 90min at 30°C
derivatizationderivatisationTRIMETHYLSILYLATION, 30min at 37°C
separationIon source temperature250°C
separationColumn length30+10m (analytical column + guard column)
separationColumn inner diameter0.25mm
separationTemperature programisotherm 1min 70°C, ramp 9°C/min, isotherm 5min 350°C
ion sourceelectron ionization70eV
detectorLECO instrument modelPegasus III TOF mass spectrometer
detectorhighest observed m/zm/z = 600
detectorlowest observed m/zm/z = 70
detectorsampling frequency20 scans/s
detectormass analyzer typeMS-TOF
deconvolutionmoving average smoothing20
deconvolutionsoftwareChromaTOF
deconvolutionsignal-to-noise ratio2
RIRetention indexdecane
RIRetention indexdodecane
RIRetention indexpentadecane
RIRetention indexoctadecane
RIRetention indexnonadecane
RIRetention indexdocosane
RIRetention indexoctacosane
RIRetention indexdotriacontane
RIRetention indexhexatriacontane
ChromatographyChromatographyAgilent GC 6890

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