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Guanosine (5TMS)


Replica Mass Spectra of Guanosine (5TMS)

replicalib entry datedetectionmethodspecies
201 February 2005 MS-TOFM[6]Reference Substance
301 February 2005 MS-TOFM[6]Reference Substance
1628 August 2012  MassBank GC 2010 Ara 
1330 March 2011  Fiehn_GC_2010 
1428 August 2012  MassBank GC 2010 Kusano 
519 July 2007 MS-TOFVAR5Reference Substance
612 May 2005  M[NIST] 
701 February 2005 MS-TOFM[6]Reference Substance
1528 August 2012  MassBank GC 2010 Tsujimoto 
819 July 2007 MS-TOFMDN35Reference Substance
14 spectrum(a)
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Spectrum Details

analyteGuanosine (5TMS)
analyte InChIInChI=1S/C25H53N5O5Si5/c1-36(2,3)28-25-27-22-19(23(31)30(25)37(4,5)6)26-17-29(22)24-21(35-40(13,14)15)20(34-39(10,11)12)18(33-24)16-32-38(7,8)9/h17-18,20-21,24H,16H2,1-15H3,(H,27,28)/t18-,20-,21-,24-/m1/s1
analyte mass644.15
citationWagner C, Sefkow M, Kopka J (2003) Phytochemistry 62, 887-900
authorsKopka J, Max Planck Institute of Molecular Plant Physiology, Department of Molecular Plant Physiology (Prof. Willmitzer L), Am Muehlenberg 1, D-14476 Golm, Germany
lib entry date01 November 2001
metabolite roleMETB
retention time (sec) 
retention index (VAR5 method, n-alkanes C10–C36)2.761,12 (predicted, according to Strehmel, N. et.al)
base peak (m/z)73
maximal intensity999
mass-intensity-peaks cardinality229 intensities
minimal m/z70
maximal m/z482
download JCAMP DXSpectrumJcampDx.ashx?id=2b7dd994-c20f-4f3f-840f-39872ed3c3d4
download MSPSpectrumMsp.ashx?id=2b7dd994-c20f-4f3f-840f-39872ed3c3d4


citationWagner C, Sefkow M, Kopka J (2003) Phytochemistry 62, 887-900
deconvolutionAMDIS, National Institute of Standards and Technology, Gaithersburg, MD, USADIRECTION:non scanning; WIDTH: 20; SUBTRACTION: two; RESOLUTION: high; SENSITIVITY: medium; SHAPE: high
derivatizationMEOX; TMSMethoxyamination:60min37°C; Trimethylsilylation:>60min37°C
detectorMS-TOFMZ:70-600; SCANS:6/s; Pegasus II TOF-MS; LECO, St. Joseph, MI, USA
extractionmethanol:water (4:1; v/v); 15min70°C 
ion sourceEI70eV
metabolic inactivationcut; liquid nitrogen; -80°C 
partitioningmethanol fraction of a methanol:water:chloroform (approx. 2:2:1, v/v/v) partitioning 
RIALKANEC12, C15, C19, C22, C28, C32, C36
separationGCCOLUMN:5%diphenyl-95%dimethylpolysiloxane (arylene), 30+10m, ID:0.25mm, DF:0.25microm, Rtx-5SilMS Integra-Guard, #NA (Restek, Bad Homburg, Germany); PROGRAM:iso2min80°C, ramp15°C/min, iso2min350°C; FLOW:Helium, 1.0ml/min; INJECTION:1microL, splitless, 230°C, 2min110psi; TRANSFER:250°C; IONSOURCE:200°C

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