How would you rate the quality of this mass spectrum?
poor, not helpful 
 outstanding, most helpful.
This spectrum was 0 times rated; on average=0.0

Zeatin, 9-beta-ribofuranosyl-, trans- (4TMS)


Replica Mass Spectra of Zeatin, 9-beta-ribofuranosyl-, trans- (4TMS)

replicalib entry datedetectionmethodspecies
126 November 2004  M[FAME4090]Standard
228 June 2002 MS-QuadM[7]Standard
427 October 2009 MS-TOFVAR5Reference Substance
3 spectrum(a)
rotate: click+drag; translate: alt+click+drag; zoom: mousewheel; save: mol

Spectrum Details

analyteZeatin, 9-beta-ribofuranosyl-, trans- (4TMS)
analyte InChIInChI=1S/C27H53N5O5Si4/c1-20(16-33-38(2,3)4)14-15-28-25-22-26(30-18-29-25)32(19-31-22)27-24(37-41(11,12)13)23(36-40(8,9)10)21(35-27)17-34-39(5,6)7/h14,18-19,21,23-24,27H,15-17H2,1-13H3,(H,28,29,30)/b20-14+/t21?,23-,24-,27-/m1/s1
analyte mass640.08
authorsStrehmel N, Kopka J, Max Planck Institute of Molecular Plant Physiology, Department of Molecular Plant Physiology (Prof. Willmitzer L), Am Muehlenberg 1, D-14476 Golm, Germany
lib entry date23 September 2009
metabolite role 
retention time (sec)1,814.36
retention index (VAR5 method, n-alkanes C10–C36)3.236,25 (observed)
base peak (m/z)73
maximal intensity109.471
mass-intensity-peaks cardinality398 intensities
minimal m/z70
maximal m/z598
download JCAMP DXSpectrumJcampDx.ashx?id=b82d6baf-951d-4475-a3f6-e24911dd2f20
download MSPSpectrumMsp.ashx?id=b82d6baf-951d-4475-a3f6-e24911dd2f20


citationKopka J, Max Planck Institute of Molecular Plant Physiology, Department of Molecular Plant Physiology (Prof. Willmitzer L), Am Muehlenberg 1, D-14476 Golm, Germany
deconvolutionChromaTOFOFFSET: "just above noise"; SMOOTHING: 20; PEAK WIDTH: 6; S/N: 2
derivatizationMEOX; TMSMETHOXYAMINATION: 90min at 30°C; TRIMETHYLSILYLATION: 30min at 37°C
detectorMS-TOFm/z = 70-600; scans:20/s; Pegasus III TOF mass spectrometer
extractionwater:chloroform (2:1; v/v) 15min at 70°C
gas chromatographAgilent GC 6890 
ion sourceEI70eV
mass spectrometry instrumentPegasus III TOF mass spectrometer 
metabolic inactivationshock-frozen in liquid nitrogen 
RIALKANEC10, C12, C15, C18, C19, C22, C28, C32, C36
separationGCCOLUMN:5%phenyl-95%dimethylpolysiloxane, 30+10m, ID:0.25mm, DF:0.25µm, 5PDM VF-5ms (Varian, Darmstadt, Germany); PROGRAM:iso1min 70°C, ramp 9°C/min, iso 5min 350°C; FLOW:Helium, 0.6mL/min; INJECTION:1µL, splitless, 230°C; TRANSFER:250°C; IONSOURCE:250°C
extractionwater chloroform(2:1; v/v) 15min at 70°C
derivatizationderivatisationMETHOXYAMINATION, 90min at 30°C
derivatizationderivatisationTRIMETHYLSILYLATION, 30min at 37°C
separationIon source temperature250°C
separationColumn length30+10m (analytical column + guard column)
separationColumn inner diameter0.25mm
separationTemperature programisotherm 1min 70°C, ramp 9°C/min, isotherm 5min 350°C
ion sourceelectron ionization70eV
detectorLECO instrument modelPegasus III TOF mass spectrometer
detectorhighest observed m/zm/z = 600
detectorlowest observed m/zm/z = 70
detectorsampling frequency20 scans/s
detectormass analyzer typeMS-TOF
deconvolutionmoving average smoothing20
deconvolutionsignal-to-noise ratio2
RIRetention indexdecane
RIRetention indexdodecane
RIRetention indexpentadecane
RIRetention indexoctadecane
RIRetention indexnonadecane
RIRetention indexdocosane
RIRetention indexoctacosane
RIRetention indexdotriacontane
RIRetention indexhexatriacontane
ChromatographyChromatographyAgilent GC 6890

comments on this mass spectrum

Post a Comment
service last updated 17/02/2017 © 2008-2014 Golm Metabolome Database - All rights reserved