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Glutamic acid (2TMS)

 

Replica Mass Spectra of Glutamic acid (2TMS)

replicalib entry datedetectionmethodspecies
201 October 2001 MS-TOFM[EIGTMS]Saccharomyces cerevisiae
609 December 2004 MS-TOFM[6]Oryza sativa
2 spectrum(a)
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Spectrum Details

analyteGlutamic acid (2TMS)
analyte InChI
analyte mass296
chromatogram4324GX59
citation 
authorsKopka J, Max Planck Institute of Molecular Plant Physiology, Department of Molecular Plant Physiology (Prof. Willmitzer L), Am Muehlenberg 1, D-14476 Golm, Germany
lib entry date09 December 2004
metabolite roleIS (QUAN)
retention time (sec)956.27
retention index (VAR5 method, n-alkanes C10–C36)1,525.46 (predicted, according to Strehmel, N. et.al)
base peak (m/z)88
maximal intensity999
mass-intensity-peaks cardinality96 intensities
minimal m/z70
maximal m/z283
download JCAMP DXSpectrumJcampDx.ashx?id=d097f8e2-406e-4b7c-9e2e-b92e2d5c8fba
download MSPSpectrumMsp.ashx?id=d097f8e2-406e-4b7c-9e2e-b92e2d5c8fba

GC-Method

nameM[6]
citationErban A, Schauer N, Fernie AR, Kopka J (2006) In: Weckwerth W (ed): Methods in Molecular Biology Vol., Humana Press Inc., Totowa, USA
attributetextdetails
deconvolutionChromaTOF V1.00 (Pegasus Driver 1.61), LECO, St. Joseph, MI, USAOFFSET:1_SMOOTHING:3_WIDTH:6_SN:>2
derivatizationMEOX; TMSMethoxyamination:90min40°C; Trimethylsilylation:45min40°C
detectorMS-TOFMZ:70-600; SCANS:20/s; Pegasus III TOF-MS; LECO, St. Joseph, MI, USA
extractionmethanol:water (4:1; v/v); 15min70°C 
ion sourceEI70eV
metabolic inactivationcut; liquid nitrogen; -80°C 
partitioningmethanol fraction of a methanol:water:chloroform (approx. 2:2:1, v/v/v) partitioning 
RIALKANEC12, C15, C18, C19, C22, C28, C32, C36
separationGCCOLUMN:5%diphenyl-95%dimethylpolysiloxane (arylene), 30+10m, ID:0.25mm, DF:0.25microm, Rtx-5SilMS Integra-Guard, #525560 (Restek, Bad Homburg, Germany); PROGRAM:iso1min70°C, ramp9°C/min, iso5min350°C; FLOW:Helium, 0.6ml/min; INJECTION:1microL, splitless, 230°C; TRANSFER:250°C; IONSOURCE:250°C

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