GOLM METABOLOME DATABASE

Mining for metabolic responses to long-term salt stress: a case study on the model legume Lotus japonicus (C)

see the experiment description.

Choose a data normalisation

The controls below visualise relative metabolite concentrations normalised to fresh weight and internal standards. Please choose from the normalisation radio buttons below to visualise this experiment with respect to different reference points. You might use the mouse wheel to zoom the controls or drag and drop to move the zoomed area. This page is undergoing active development and might change without further notice in the future.


relative to replica group

Please choose from the 10 normalisations for data visualisation!

replica group correlations

The correlation and network plots below visualise the statistical relationship between the replica groups based on the oberserved metabolite profiles. The network plot is derived from the correlation such that only positive correlations are converted into distances, Distance(i,j) = 1 - COR(i,j). The edges are filtered for distances < 0.5. The plot can be zoomed and dragged by using the mouse wheel and left mouse button.

technical replicates (aka chromatogram) correlations

This correlation plot visualise the statistical relationship between the chromatograms based on the observed metabolite profiles. It is kind of 'zoom in' into the replica groups. The assumtion is, that chromatograms within one replica group correlate very well. However, the correlation of chromatograms between replica groups is much worse, depending on the similarity of the replica groups.

metabolite correlations

The correlation and network plots below visualise the statistical relationship between the identified metabolites based on their observed profile accross replica groups. The network plot is derived from the correlation such that only poistive correlations are converted into distances, Distance(i,j) = 1 - COR(i,j). The edges are filtered for distances < 0.2. The plot can be zoomed and dragged by using the mouse wheel and left mouse button.

dendrogram view

The heatmap below can be zoomed and dragged by using the mouse wheel and left mouse button, respectively! Please note, that the order of columns in the control below does neither necessarily reflect the numerical ordering of the replica group column names nor correspond to the column order of the second heatmap.

sortable table based heatmap with boxplot extension

The table below can be sorted by clicking the column headers. Metabolite links point to the metabolite details pages. The table is initially sorted according to decreasing anova F-score values (metabolites with large changes in relative concentration between the replica groups at the top and metabolites with small concentration changes between replica groups at the bottom). Please hoover the mouse over the table to see box plots of the metabolite concentrations according to the replica groups.
metaboliteclassrg1 Salt:0mM
details		rg2 Salt:50mM
details		rg3 Salt:100mM
details		rg4 Salt:150mM
details		rg5 Salt:25mM
details		rg6 Salt:50mM
details		rg7 Salt:75mM
details		varianceanova F score
ProlineAcid (Amino)777.62201.40
OnonitolPolyol (Methoxyinositol)727.45148.99
Malic acidAcid (Hydroxy)742.52142.60
Phosphoric acidAcid (Phosphate)594.7186.48
Glutaric acid, 3-hydroxy-3-methyl-na526.2683.49
Malonic acidAcid (Dicarboxylic)648.5570.23
Maleic acidAcid (Dicarboxylic)678.6955.09
GlycineAcid (Amino)409.2952.89
Succinic acidAcid (Dicarboxylic)465.8548.87
Glutamic acidAcid (Amino)543.7041.49
Gulonic acidAcid (Hexonic)710.7534.39
XyloseSugar (Pentose, aldose)269.1233.53
Fumaric acidAcid (Dicarboxylic)449.6833.21
myo-InositolPolyol (Inositol)351.4830.39
Erythronic acidAcid (Hydroxy)242.0829.35
MannitolPolyol (Hexitol)303.3327.93
Glucose-6-phosphateSugar (Phosphate)388.3326.19
GlutamineAcid (Amino)792.5226.07
Cinnamic acid, 4-hydroxy-, cis-Acid (Phenylpropanoic)354.1524.98
PinitolPolyol (Methoxyinositol)282.1124.83
Pyroglutamic acidLactam376.1023.60
Phosphoric acid monomethyl esterAcid (Phosphate)522.7123.09
Threonic acidAcid (Hydroxy)254.7420.32
Saccharic acidAcid (Hexaric)298.8419.42
Glucuronic acidAcid (Hexuronic)562.5718.80
Shikimic acidAcid (Hydroxy)NaNNaNNaNNaN421.8017.42
RiboseSugar (Pentose, aldose)139.4215.90
Butanoic acid, 2,4-dihydroxy-Acid (Hydroxy)194.4815.39
Citric acidAcid (Tricarboxylic)589.1414.26
PsicoseSugar (Hexose, ketose)141.8313.96
Cinnamic acid, 4-hydroxy-, trans-Acid (Phenylpropanoic)NaNNaN746.2313.46
SucroseSugar (Disaccharide)97.7913.38
PhenylalanineAcid (Amino)329.2612.68
MaltoseSugar (Disaccharide)679.9312.14
Arabinonic acidna243.6211.95
Malic acid, 2-methyl-Acid (Hydroxy)446.3311.37
SpermidineAmine (Poly)685.8410.65
Trehalose, alpha,alpha'-Sugar (Disaccharide)247.3910.60
AsparagineAcid (Amino)933.519.82
LeucineAcid (Amino)226.529.51
ThreonineAcid (Amino)473.608.84
Glucose, 1,6-anhydro, beta-D-Sugar (Hexose, aldose, anhydride)176.388.44
Galactonic acidAcid (Hexonic)201.048.16
IsoleucineAcid (Amino)216.448.06
GalactinolConjugate (Hexosyl, Inositol)301.137.96
ArabitolPolyol (Pentitol)421.247.67
TyrosineAcid (Amino)807.557.39
SerineAcid (Amino)515.977.39
ThreitolPolyol (Tetraol)106.586.83
Galactaric acidAcid (Hexaric)170.786.34
Threonic acid-1,4-lactoneAcid (Hydroxy, lactone)167.726.29
CarbodiimideImide239.126.21
AlanineAcid (Amino)273.586.14
Erythronic acid-1,4-lactoneAcid (Hydroxy, lactone)134.866.11
FructoseSugar (Hexose)462.805.02
Alanine, 3-cyano-Acid (Amino)514.934.95
Piperidine-2-carboxylic acidAcid (N-heterocycle)281.514.73
MannoseSugar (Hexose, aldose)149.254.46
Glycolic acidna117.553.67
cis-Aconitic acidAcid (Tricarboxylic)300.663.53
Dehydroascorbic acidAcid (Hydroxy)267.913.50
Benzoic acidAcid (Aromatic)56.083.31
GlucoseSugar (Hexose, aldose)321.533.19
Uric acidPurine358.643.17
Aspartic acidAcid (Amino)258.623.06
SorbitolPolyol (Hexitol)379.482.97
beta-D-Galactopyranoside, 1-isopropyl-, 1-thio-naNaN342.992.90
ValineAcid (Amino)241.892.78
GlycerophosphoglycerolPolyol (Phosphate)356.272.64
EthanolamineAlcohol (Amino)55.012.57
HydroxylamineAmine (Hydroxy)330.872.40
Glyceric acidAcid (Hydroxy)147.102.33
Oxalic acidAcid (Dicarboxylic)670.872.22
PhenethylamineAmine (Aromatic)116.962.21
AdenosineNucleoside245.872.01
GlycerolPolyol (Triol)271.631.92
Hydrogen sulfideAcid (Thiol)372.171.81
ErythritolPolyol (Tetraol)81.701.77
Decane, n-Alkane78.361.52
DodecaneAlkane43.781.51
RaffinoseSugar (Trisaccharide)267.671.38
2-HydroxypyridineAlcohol (N-heterocycle)155.431.25
HomoserineAcid (Amino)223.031.22
Pentadecane, n-Alkane72.361.20
Lactic acidAcid (Hydroxy)186.651.10
PutrescineAmine (Poly)264.321.04
Hexadecan-1-olAlcohol (Mono, Primary)510.571.03
Palmitic acidAcid (Fatty acid trimethylsilyl ester)248.350.90
RibitolPolyol (Pentitol)1.010.83
SpermineAmine (Poly)NaN403.140.83
NonadecaneAlkane86.680.80
4-HydroxypyridineAlcohol (N-heterocycle)154.350.80
Docosane, n-Alkane96.130.76
Benzoic acid, 4-hydroxy-Acid (Aromatic)289.690.75
Stearic acidAcid (Fatty acid trimethylsilyl ester)516.970.69
Octadecane, n-Alkane91.250.68
GalactoseSugar (Hexose, aldose)92.140.66
DiethyleneglycolAlcohol (Ether)359.160.61
LysineAcid (Amino)157.230.54
Itaconic acidAcid (Dicarboxylic)NaN225.670.53
Nicotinic acidAcid (N-heterocycle)448.700.45
Ferulic acid, E-Acid (Phenylpropanoic)192.500.27
3-HydroxypyridineAlcohol (N-heterocycle)183.530.26
Octacosane, n-Alkane217.450.26
Dotriacontane, n-Alkane563.250.15
105 metabolite(s) in 7 replica group(s).
1.1901 
98.8444

Excluded Metabolites

Internal Standards

Referenced Chromatograms

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service last updated 31/08/2021 © 2008-2014 Golm Metabolome Database - All rights reserved
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